Acetylcholine is found in your central nervous system (CNS), your brain and spinal cord, as well as in your peripheral nervous system (the nerves that branch out from your CNS and connect with all other parts of your body, including muscles and organs). Acetylcholine is released both in your central nervous system (CNS), your brain and spinal cord, as well as in your peripheral nervous system (the nerves that branch out from your CNS and connect with all other parts of your body, including muscles and organs). In your brain, acetylcholine originates from two major places: the basal forebrain and the mesopontine tegmentum area. In your peripheral nervous system, acetylcholine is released into the neuromuscular junction. This is where nerves meet muscle cells. Acetylcholine also plays a significant role in your autonomic nervous system, a branch of your peripheral nervous system. Your autonomic nervous system regulates many of the automatic functions of your body, such as the proper functioning of your internal organs. Acetylcholine is also involved in your immune system and is secreted by T lymphocytes. T lymphocytes are a type of body-protective white blood cell.