Yes, medical ozone therapy has been shown to raise
AMPK (AMP-activated protein kinase) levels.<BR> Research indicates that low-concentration ozone acts as a moderate stressor that triggers the body's endogenous protective mechanisms, leading to increased AMPK phosphorylation. <BR>
Mechanisms and Effects of Ozone-Induced AMPK Activation<BR>

    Activation Pathway: Ozone increases the production of reactive oxygen species (ROS) and hydrogen peroxide <BR>(

), which can directly activate AMPK or lead to a decrease in the ATP-to-AMP ratio, signaling the cell's "energy sensor" to turn on.
Anti-Inflammatory Action:<BR> Activated AMPK triggers the AMPK/Gas6-MerTK/SOCS3 signaling pathway.<BR> This process:

    Promotes the clearance of apoptotic (dying) cells by macrophages.<BR>
    Upregulates SOCS3, an anti-inflammatory factor that suppresses pro-inflammatory cytokines like IL-1β, IL-6, and TNF-α.<BR>

Pain Management: Peri-sciatic and subcutaneous ozone injections have been shown to alleviate neuropathic and inflammatory pain <BR>specifically through this AMPK activation.
Dose Sensitivity:<BR> The effect is highly dose-dependent. Low concentrations<BR> (e.g., 20 30 ug mL) typically activate beneficial pathways, while excessively high doses (e.g., 45 ug mL and above) <BR>may induce severe oxidative stress that can overwhelm these protective responses. 

Therapeutic Applications Related to AMPK<BR>

    Neuropathic Pain: Studies on rat models of nerve injury<BR> found that ozone's analgesic effects were completely blocked when an AMPK inhibitor was used,<BR> proving that AMPK is necessary for the treatment's success.
    Gout and Arthritis: Ozone has been found to reduce joint inflammation and relieve gout pain by upregulating the same AMPK-mediated pathways.
    Organ Protection: Research suggests ozone-induced AMPK activation may protect against sepsis-induced lung injury and chemotherapeutic enteritis by enhancing tissue